进一步提高血清促甲状腺激素（TSH）检测的敏感性
    增强化学发光酶免疫法测定血清促甲状腺激素方法的建立及初步应用
 通过正交试验，并经方差分析，获得增强发光酶免疫检测法的*实验条件，将其与TSH-酶联免疫吸附测定（ELISA）试剂盒相结合，建立TSH的增强化学发光酶免疫检测法

To improve the sensitivity of measurement of TSH in serum.Methods　Establishment and application of a new chemiluminescence enzyme immunoassay to determine human thyrotropin in serum 　LIANG Yan，LIN Hanhua，WANG Muti. Department of Pediatrics, Tongji Hospital,Tongji Medical University

The authors developed a new enhanced chemiluminescence enzyme immunoassay （ECLIA）, which combined luminol-H2O2-HRP system enhanced by para-iodophenol（PIP） with TSH-ELISA test kit. The optimal experimental conditions of the enhanced chemiluminescence were obtained by orthogonal design and analysis of variance
材料和方法
仪器和试剂：Metrohm Herisau E350B-pH计（瑞士）。鲁米诺 （Luminol,Serva） 用pH 10.3、0.05 mol/L 碳酸盐缓冲液配制成5×10-2 mol/L储备液放置3天后再以pH 8.2、0.1 mol/L 碳酸氢钠-氢氧化钠缓冲液稀释备用。

将化学发光技术与TSH-ELISA试剂盒相结合，建立了TSH的增强化学发光酶免疫检测法