简要描述:
亚硫酸铁琼脂, 我公司专业销售优质培养基产,质量保证,*,订购!
更新时间:2024-08-26;厂商性质:经销商;览量:697
亚硫酸铁琼脂由于这类培养基成分复杂,制备吋很微小的偏差都会对培养基的性能造成很大的影响,因此,使用商品化的脱水培养基和制备好的抗生素补充液。
中文名称:亚硫酸铁琼脂
Sulfite Iron Agar
别名:
规格:BR
包装:250g/瓶
用途:用于细菌总数测定、纯培养及保存菌种,也可用于消毒效果测定;用于沙门氏菌培养(GB)
性状:有各种不同的配方,如牛肉膏粉90g、蛋白胨270g、食盐136g、琼脂粉520g、无水碳酸钠9g。易潮解。加水煮沸溶化后呈浅黄色半透明液体,pH7.4(±0.2),冷至45℃开始凝固。 称取本品33g,加入蒸馏水或去离子水1 L,搅拌加热煮沸至*溶解, 分装试管或三角瓶,121℃高压灭菌15min备用。
贮存 旋紧瓶盖,贮存于避光、阴凉干燥处。
抗生素的平板抑菌实验结果
将万古霉素、头孢噻吩、红霉素分别以不同的浓度添加到基础培养基(脑心琼脂)中,接种定量菌液,观察抑菌效果,以确定zui终的抗生素组合和添加浓度。结果表明,万古霉素、头孢噻吩适合使用于阪崎肠杆菌选择性培养,与定量抑菌实验结果*;而红霉素与定量抑菌浓度实验结果不*,不适合使用于阪崎肠杆菌选择性培养。
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